Rapid and Sensitive Detection of Apple stem pitting virus in Apple Trees Through RNA Amplification and Probing with Fluorescent Molecular Beacons
نویسندگان
چکیده
منابع مشابه
شناسایی مولکولی و گروه بندی جدایه های دو ویروسapple stem grooving virus و apple stem pitting virus در استان اصفهان
چکیده سیب درختی (malus domestica borkh) متعلق به خانواده rosaceae از میوه های مهم و اقتصادی مناطق معتدله و سردسیری ایران است. ویروس ساقه شیاری سیب apple stem grooving virus (asgv) عضو تیپ جنس capillovirus و ویروس ساقه آبله ای سیب apple stem pitting virus (aspv) عضو تیپ جنس foveavirus متعلق به خانوادهbetaflexiviridae ، از مهمترین ویروس های بیماری زای سیب محسوب می شوند. آلودگی این دو ویروس درس...
15 صفحه اولGenetic Diversity of a Natural Population of Apple stem pitting virus Isolated from Apple in Korea
Apple stem pitting virus (ASPV), of the Foveavirus genus in the family Betaflexiviridae, is one of the most common viruses of apple and pear trees. To examine variability of the coat protein (CP) gene from ASPV, eight isolates originating from 251 apple trees, which were collected from 22 apple orchards located in intensive apple growing areas of the North Gyeongsang and North Jeolla Provinces ...
متن کاملoccurrence, distribution, and molecular characterization of apple stem pitting virus in iran
apple stem pitting virus (aspv) is one of the most common viruses of apple and pear trees. during 2012 to 2013, a total of 1053 symptomatic and asymptomatic leaf samples were collected from orchards in nine provinces of iran. aspv infection was detected by das-elisa and pcr in 54 samples (5.12%) from seven provinces. based on the geographical origin, 22 representative isolates were selected for...
متن کاملSequence diversity and potential recombination events in the coat protein gene of Apple stem pitting virus.
The variability of the Apple stem pitting virus (ASPV) coat protein (CP) gene was investigated. The CP gene of ten virus isolates from apple and pear trees was sequenced. Comparison of all sequenced virus isolates revealed high diversity of the CP gene (70.7-93.5% at the nucleotide level and 77.8-98.7% at the amino acid level). Additionally, one or two deletions in the N-terminal part of the co...
متن کاملEnzymatic signal amplification of molecular beacons for sensitive DNA detection
Molecular beacons represent a new family of fluorescent probes for nucleic acids, and have found broad applications in recent years due to their unique advantages over traditional probes. Detection of nucleic acids using molecular beacons has been based on hybridization between target molecules and molecular beacons in a 1:1 stoichiometric ratio. The stoichiometric hybridization, however, puts ...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2001
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto.2001.91.11.1085